Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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At that time, the early indicators have been dismissed as particles, however the retrospective analysis demonstrated shared properties Along with the problematic cells in tradition. Tracing the an infection aided the lab create more laboratory methods for screening and protection of assay-significant mobile lines.
As samples are obtained about the Attune CytPix Flow Cytometer, the higher-pace brightfield digicam captures and retailers images of detected events in a fee as many as 6,000 images per second, dependant upon the flow amount and picture dimensions. For higher flexibility, Attune Cytometric Application helps you to change the impression seize frequency as needed and select a specific gate for picture seize, if required.
Blend acoustic concentrating technological know-how with substantial-parameter Investigation for versatile spectral unmixing and traditional workflows.
Discover new insights into your experimental data by visualizing populations utilizing combined fluorescence and morphology data. Our software interface wizards empower downsampling, concatenation (merged information) and dimensionality instruments that are crafted into your software program for an intuitive, streamlined info Evaluation expertise.
"An image isn't very really worth a thousand dots, nevertheless it seriously helps to see visuals. It is great to view photos that correlate with staining. As an example, AnnexinV-beneficial cells which are morphologically distinct than healthy cells, or CD14+ monocytes which are greater and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Furthermore, it really is illuminating to find out what number of doublets slip via classic singlet gates, and great to validate the position of unanticipated double-good functions in my Examination."
Optimizing gating strategy. Even strong manual singlet gating is mistake-susceptible and remains a subjective conclusion level in Pretty much all flow cytometry assays. Imaging may be used to verify and adjust gates to incorporate only solitary cells of curiosity.
Our researchers will layout panels to suit your needs as persistently you need—totally free, no product invest in wanted.
The Attune NxT cytometer could be the trustworthy workhorse instrument your lab can trust in for constant and trusted overall performance. The Attune CytPix product encompasses a higher-speed brightfield digicam and AI-pushed graphic Evaluation software, with new updates including machine Finding out instruments and an intuitive user interface, enabling Increased info precision, precision, and a lot quicker insights into sample biology.
Users can perspective function photographs in real-time to substantiate sample high quality, enhance protocols, and file the morphology of each and every mobile population determined for analysis. Imaging capture also Added benefits from acoustic concentrating, which really helps to situation the cells to make sure that a pointy, centered impression is attained.
Seem waves help precise cell alignment, causing Excellent data good quality and ~10X more quickly sample flow premiums.
Application Observe: Automatic image Investigation decreases person-to-user variability in flow cytometry gating methods
Constructed-in dimensionality reduction resources increased resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm anticipated inhabitants resolution in lysed entire human blood. Person can check out occasions from Each individual cluster for Visible confirmation of cell phenotypes.
Versatile payment system from Agilent Monetary Methods help you receive the crucial instrumentation you will need for rapid task, or to expand your lab.
Gating on these extended parameters lets you immediately and accurately establish populations of fascination and confirm gating strategy with little or no manual critique. The Attune software program provides usage of point out-of-the-art equipment Understanding Investigation applications within just an expertly intended user interface, rendering it extremely user friendly.
Because the mobile line had Formerly been analyzed over the Attune CytPix Flow Cytometer, the scientists went back to the images and were being in the position to doc the microbial infection a minimum of 5 days before.
Agilent Value Assure assures you a minimum of a decade of instrument use in the date you buy. Or else, We're going to credit history you Along with the residual value of the https://chancekxkvg.wikipublicist.com/5132573/flow_cytometer_a_revolutionary_tool_for_cell_analysis procedure toward an upgraded model.
What seemed to be a homogeneous population is in fact much more varied—an insight that needs to be viewed as when interpreting results.